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M9640557.TXT
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1996-03-04
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Document 0557
DOCN M9640557
TI Diagnosis of microsporidial keratitis by confocal microscopy and the
chromatrope stain.
DT 9604
AU Shah GK; Pfister D; Probst LE; Ferrieri P; Holland E; Department of
Ophthalmology, University of Minnesota Hospital; 55455-0501, USA.
SO Am J Ophthalmol. 1996 Jan;121(1):89-91. Unique Identifier : AIDSLINE
MED/96142051
AB PURPOSE: To illustrate the value of confocal microscopy and chromatrope
stain in the diagnosis of microsporidial keratitis. METHODS: In vivo
confocal microscopy was performed on a man with the human
immunodeficiency virus who had severe bilateral epithelial keratitis
refractory to topical antibacterial medications. The results were
compared to conjunctival scrapings stained with the chromatrope-based
Weber stain. RESULTS: Confocal microscopy demonstrated many small,
intraepithelial opacities of the corneal epithelium, which were
suggestive of Microsporidia. Results of the chromatrope stain of
conjunctival scrapings confirmed the diagnosis of microsporidial
keratitis. CONCLUSIONS: Rapid diagnosis allowed prompt initiation of
topical fumagillin, which permitted rapid, long-term control of the
symptoms of microsporidial keratitis.
DE Adult Animal Antiprotozoal Agents/THERAPEUTIC USE AIDS-Related
Opportunistic Infections/*DIAGNOSIS/DRUG THERAPY/ PARASITOLOGY Case
Report Conjunctiva/DRUG EFFECTS/PARASITOLOGY Cornea/DRUG
EFFECTS/PARASITOLOGY Dyes Epithelium/DRUG EFFECTS/PARASITOLOGY Eye
Infections, Parasitic/*DIAGNOSIS/DRUG THERAPY/PARASITOLOGY Fatty Acids,
Unsaturated/THERAPEUTIC USE Human Keratitis/*DIAGNOSIS/DRUG
THERAPY/PARASITOLOGY Male Microscopy, Confocal/*METHODS Microspora
Infections/*DIAGNOSIS/DRUG THERAPY/PARASITOLOGY Microsporida/*ISOLATION
& PURIF JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).